Abstract :
Aim of the Study: Carbapenem resistance in Acinetobacter baumannii has become a major concern for treating physicians. The reason for this study was to identify the predominance of different beta-lactamases among multi-drug resistant Acinetobacter baumannii from clinical specimens.
Methods: October 2024 to May 2024 marked the conduct of a cross-sectional survey. Thirty-three non-repetitive A. baumannii confines resistant to carbapenem were gathered. Testing for AST (Antimicrobial Susceptibility Testing) was done using the Kirby-Bauer disk diffusion technique. The E-test method demonstrated MICs. The MHT (Modified Hodge Test) was used to determine whether carbapenemase production was detected in the tests. Using the EDTA-disk synergy test, the MBL (Metallo-Β-Lactamases) in the carbapenem-resistant segregates of A. baumannii were examined.
Results: All isolates exhibited resistance to multiple drugs and antimicrobial agents, such as carbapenems, but they were susceptible to colistin. The Modified Hodge test confirmed carbapenemase production in 31 (93.93%) of these confines. The phenotypic strategy revealed that 18 (or 54%) of the separates generated MBL.
Conclusion: According to the results of our investigation, one of the main issues is the high prevalence of MBL-generating Acinetobacter baumannii isolates that are resistant to carbapenem. Strict measures for infection control are required in this situation. Hence, it is significant to limit the emergence of pathogens that cause resistance by using the proper anti-microbial approach and limiting the indiscriminate use of carbapenems.

Keyword :Acinetobacter baumannii, Carbapenem resistance, Metallo-beta-lactamases, Modified hodge test.